clone 80h3  (Bio-Rad)

Journal: STAR Protocols

Article Title: An optimized protocol for phenotyping human granulocytes by mass cytometry

doi: 10.1016/j.xpro.2022.101280

Figure Lengend Snippet: Optimization of eosinophil and neutrophil detection (A) Cells were treated with RBC lysis buffer, followed by staining with surface antibodies (including CD16 and CD66b) prior to fixation with PFA as outlined in protocol. (B) Cells were treated with RBC lysis buffer, fixed with PFA, and then stained with surface antibodies. (C) Cells were treated with a commercially available RBC lysis/fix solution prior to staining with surface antibodies.

Article Snippet: CD66b (Clone 80H3) - 152Sm , Fluidigm Sciences , Cat#3152011B.

Techniques: Red Blood Cell Lysis, Staining

Journal: STAR Protocols

Article Title: An optimized protocol for phenotyping human granulocytes by mass cytometry

doi: 10.1016/j.xpro.2022.101280

Figure Lengend Snippet:

Article Snippet: CD66b (Clone 80H3) - 152Sm , Fluidigm Sciences , Cat#3152011B.

Techniques: Recombinant, Blocking Assay, Electron Microscopy, Red Blood Cell Lysis, Antibody Labeling, Membrane, Software

Journal: STAR Protocols

Article Title: An optimized protocol for phenotyping human granulocytes by mass cytometry

doi: 10.1016/j.xpro.2022.101280

Figure Lengend Snippet: Mass cytometry surface antibody panel

Article Snippet: CD66b (Clone 80H3) - 152Sm , Fluidigm Sciences , Cat#3152011B.

Techniques: Mass Cytometry

Journal: Liver Cancer

Article Title: Increased Expression of Programmed Death-Ligand 1 in Infiltrating Immune Cells in Hepatocellular Carcinoma Tissues after Sorafenib Treatment

doi: 10.1159/000489021

Figure Lengend Snippet: Programmed death-ligand 1 (PD-L1) expression in tumor-infiltrating immune cells (a) and tumor cells (b) in paired hepatocellular carcinoma tissues before and after sorafenib treatment.

Article Snippet: The slides were then incubated with primary antibodies, including anti-PD-L1 antibody clone SP142 (1: 50, Spring Bioscience, Pleasanton, CA, USA), anti-human CD3 (1: 100, clone 2GV6; Ventana, Tuscon, AZ, USA), CD66b (1: 100, clone 80H3, OriGene, Rockville, MD, USA), CD68 (1: 100, clone KP1, Ventana, Tuscon, AZ, USA), and CD163 (1: 100, clone 10D6; Thermo Fisher Scientific, CA, USA), at 4°C overnight.

Techniques: Expressing

Journal: Liver Cancer

Article Title: Increased Expression of Programmed Death-Ligand 1 in Infiltrating Immune Cells in Hepatocellular Carcinoma Tissues after Sorafenib Treatment

doi: 10.1159/000489021

Figure Lengend Snippet: Kaplan-Meier curves of overall survival (a, c) and time to treatment discontinuation (b, d) of advanced hepatocellular carcinoma patients with low programmed death-ligand 1 (PD-L1) expression (IHC 0/1) and high PD-L1 expression (IHC 2/3) in tumor-infiltrating immune cells (ICs) before (a, b) and after (c, d) sorafenib treatment.

Article Snippet: The slides were then incubated with primary antibodies, including anti-PD-L1 antibody clone SP142 (1: 50, Spring Bioscience, Pleasanton, CA, USA), anti-human CD3 (1: 100, clone 2GV6; Ventana, Tuscon, AZ, USA), CD66b (1: 100, clone 80H3, OriGene, Rockville, MD, USA), CD68 (1: 100, clone KP1, Ventana, Tuscon, AZ, USA), and CD163 (1: 100, clone 10D6; Thermo Fisher Scientific, CA, USA), at 4°C overnight.

Techniques: Expressing

Journal: Liver Cancer

Article Title: Increased Expression of Programmed Death-Ligand 1 in Infiltrating Immune Cells in Hepatocellular Carcinoma Tissues after Sorafenib Treatment

doi: 10.1159/000489021

Figure Lengend Snippet: Factors associated with increased PD-L1 expression in tumor-infiltrating immune cells in paired HCC tissues in the multiple logistic regression model

Article Snippet: The slides were then incubated with primary antibodies, including anti-PD-L1 antibody clone SP142 (1: 50, Spring Bioscience, Pleasanton, CA, USA), anti-human CD3 (1: 100, clone 2GV6; Ventana, Tuscon, AZ, USA), CD66b (1: 100, clone 80H3, OriGene, Rockville, MD, USA), CD68 (1: 100, clone KP1, Ventana, Tuscon, AZ, USA), and CD163 (1: 100, clone 10D6; Thermo Fisher Scientific, CA, USA), at 4°C overnight.

Techniques: Expressing

Journal: Liver Cancer

Article Title: Increased Expression of Programmed Death-Ligand 1 in Infiltrating Immune Cells in Hepatocellular Carcinoma Tissues after Sorafenib Treatment

doi: 10.1159/000489021

Figure Lengend Snippet: Representative patients with advanced hepatocellular carcinoma with high expression of programmed death-ligand 1 (PD-L1) in tumor-infiltrating immune cells (ICs). Consecutive slides of the patient with peritoneal metastasis (a) and the patient with lung metastasis (b) were stained with H&E, PD-L1, CD3, CD66b, CD68, and CD163, respectively. PD-L1-expressing ICs were mainly located around the tumor-invasive margins, and their distributions were highly corresponding to the staining pattern of CD68 and CD163, but not those of CD3 and CD66b. c Consecutive slides of another patient with colonic metastasis were stained with H&E and were double-stained with PD-L1 and CD68, which were shown in low- and high-power fields. A majority of the PD-L1-positive cells (brown, membranous) around the tumor margin coexpressed CD68 (green, cytoplasmic). The dashed red lines represent the area of invasive margins. The dashed black square in c indicates the area of high-power field.

Article Snippet: The slides were then incubated with primary antibodies, including anti-PD-L1 antibody clone SP142 (1: 50, Spring Bioscience, Pleasanton, CA, USA), anti-human CD3 (1: 100, clone 2GV6; Ventana, Tuscon, AZ, USA), CD66b (1: 100, clone 80H3, OriGene, Rockville, MD, USA), CD68 (1: 100, clone KP1, Ventana, Tuscon, AZ, USA), and CD163 (1: 100, clone 10D6; Thermo Fisher Scientific, CA, USA), at 4°C overnight.

Techniques: Expressing, Staining

Journal: Scientific Reports

Article Title: The Prognostic Importance of CD20 + B lymphocytes in Colorectal Cancer and the Relation to Other Immune Cell subsets

doi: 10.1038/s41598-019-56441-8

Figure Lengend Snippet: Multispectral imaging of immune cell infiltrates in CRC tissues. ( a ) A high-magnification area (20x) of a representative multiplexed IHC stained composite image after imaging and spectral unmixing (left panel), after tissue segmentation into tumour (magenta) and stromal (blue) compartments (middle panel), and after cell phenotyping (right panel). The following colors were used to identify the different markers; pan-Cytokeratin (magenta), CD20 (yellow), CD8 (red), CD66b (green), CD68 (cyan), FoxP3 (orange), and DAPI (blue). ( b ) Illustrates the staining pattern of the individual markers on a high-resolution area of a representative TMA core, after spectral unmixing, using the pathology view tool. ( c ) Histograms displaying the distribution (number of cells per mm 2 ) of infiltrating immune cell subsets in the cohort of 275 CRC patients. In addition, the normal curve and the median number of infiltrating cells is shown.

Article Snippet: The CD4 antibody used in the kit was exchanged by CD66b (clone 80H3, LsBio, Seattle, WA, USA).

Techniques: Imaging, Staining

Journal: Scientific Reports

Article Title: The Prognostic Importance of CD20 + B lymphocytes in Colorectal Cancer and the Relation to Other Immune Cell subsets

doi: 10.1038/s41598-019-56441-8

Figure Lengend Snippet: The correlation of infiltration of CD20 positive cells in the stromal compartment to infiltration of other immune cell markers.

Article Snippet: The CD4 antibody used in the kit was exchanged by CD66b (clone 80H3, LsBio, Seattle, WA, USA).

Techniques: Marker, Significance Assay

Journal: Scientific Reports

Article Title: The Prognostic Importance of CD20 + B lymphocytes in Colorectal Cancer and the Relation to Other Immune Cell subsets

doi: 10.1038/s41598-019-56441-8

Figure Lengend Snippet: Cox regression analyses of infiltrating immune cells in predicting survival of CRC patients.

Article Snippet: The CD4 antibody used in the kit was exchanged by CD66b (clone 80H3, LsBio, Seattle, WA, USA).

Techniques: Marker, Significance Assay

Journal: Scientific Reports

Article Title: The Prognostic Importance of CD20 + B lymphocytes in Colorectal Cancer and the Relation to Other Immune Cell subsets

doi: 10.1038/s41598-019-56441-8

Figure Lengend Snippet: Prognostic relations of immune cell subsets in CRC.

Article Snippet: The CD4 antibody used in the kit was exchanged by CD66b (clone 80H3, LsBio, Seattle, WA, USA).

Techniques: Marker, Significance Assay

Journal: Scientific Reports

Article Title: The Prognostic Importance of CD20 + B lymphocytes in Colorectal Cancer and the Relation to Other Immune Cell subsets

doi: 10.1038/s41598-019-56441-8

Figure Lengend Snippet: Prognostic relation of CD20 + cells to different immune cell subsets in CRC. Cases scored for subgroups of CD20 high or low together with; ( a ) CD8 high or low; ( b ) CD66b high or low; ( c ) CD68 high or low; and ( d ) FoxP3 high or low, in the tumour stromal compartment as indicated. Shown are Kaplan-Meier plots. Log-rank tests were used to calculate P -values.

Article Snippet: The CD4 antibody used in the kit was exchanged by CD66b (clone 80H3, LsBio, Seattle, WA, USA).

Techniques:

Journal: Cell

Article Title: Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses

doi: 10.1016/j.cell.2017.04.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-Human CD66b (clone 80H3) – 152Sm , Fluidigm , Cat#: 3152011B.

Techniques: Recombinant, Labeling, Expressing, Gene Expression, Sequencing, Software